An Iml3-Chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation

Abstract

Accurate segregation of genetic material in eukaryotes relies on the kinetochore, a multiprotein complex that connects centromeric DNA with microtubules. In yeast and humans, two proteins – Mif2/CENP-C and Chl4/CENP-N – interact with specialized centromeric nucleosomes and establish distinct but cross-connecting axes of chromatin-microtubule linkage. Proteins recruited by Chl4/CENP-N include a subset that regulates chromsome transmission fidelity. We show that Chl4 and a conserved member of this subset, Iml3, both from S. cerevisiae, form a stable protein complex, which interacts with Mif2 and Sgo1. We have determined the structures of an Iml3 homodimer and an Iml3-Chl4 heterodimer, which suggest a mechanism for regulating assembly of this functional axis of the kinetochore. We propose that at the core centromere, the Chl4-Iml3 complex participates in recruiting factors, such as Sgo1, that influence sister chromatid cohesion and encourage sister kinetochore biorientation.

Stephen M. Hinshaw
Stephen M. Hinshaw
Scientist, Stanford Cancer Institute

Head gello maker.